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Image Search Results
Journal: Cell Research
Article Title: Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis
doi: 10.1038/cr.2017.11
Figure Lengend Snippet: MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around the HA/TCP (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Article Snippet: In vivo BMMSC implantation 4.0 × 10 6 BMMSCs, isolated from C57BL/6J, Tsk / + , MSCT, exosome-treated, and Ad-miR151-treated mice, were mixed with
Techniques: Staining, Cell Culture, Western Blot, Expressing, Generated